Title: | Simultaneous Capture, Detection, and Inactivation of Bacteria as Enabled by a Surface-Enhanced Raman Scattering Multifunctional Chip |
Authors: | Houyu Wang, Yanfeng Zhou, Xiangxu Jiang, Bin Sun, Ying Zhu, Hui Wang, Yuanyuan Su, and Yao He* |
Institutions: | Institute of Functional Nano & Soft Materials (FUNSOM) andCollaborative Innovation Center of Suzhou Nanoscience and Technology (NANO-CIC), Soochow University, Suzhou 215123 (China) |
Abstract: | Herein, we present a multifunctional chip based on surface-enhanced Raman scattering (SERS) that effectively captures, discriminates, and inactivates pathogenic bacteria.The developed SERS chip is made of a silicon wafer decorated with silver nanoparticles and modified with 4-mercaptophenylboronicacid (4-MPBA). It was prepared in a straightforward manner by chemical reduction assisted by hydrogen fluoride etching, followed by the conjugation of 4-MPBA through Ag-S bonds. The dominant merits of the fabricated SERS chip include excellent reproducibility with a relative standard deviation (RSD) value smaller than 11.0%, adaptable bacterial-capture efficiency (ca. 60%) at low concentrations (500–2000 CFUmL-1), a low detection limit (down to a concentration of 1.0×102 cells mL-1), and high antibacterial activity (an antibacterial rate of ca. 97%). The SERS chip enabled sensitive and specific discrimination of Escherichia coli and Staphylococcus aureus from human blood. |
IF: | 11.261 |
Link: | http://onlinelibrary.wiley.com/doi/10.1002/anie.201412294/epdf Editor: Danting Xiang |