A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR), and semi-quantitatively, i.e. above/below a certain amount of DNA molecules (semi quantitative real-time PCR).
Instrument name | Real-Time PCR Detection System |
Model number | CFX Connect |
Manufacturer | BIO-RAD |
Specifications | 1. Channel number: 3; 2 target genes and 1 FRET channels can be detected simultaneously. 2. Excitation light source: high intensity LED lamp; Detector: PTD. 3. Excitation / emission wavelength range: 450-580 nm. 4. Sample number: 96 well; Sample carrier: 96 well plate, pipe, PCR pipe. 5. Sample Volume: 1-50µl. 6. Suitable for a variety of fluorescence methods: Taqman, Molecular Beacon, FRET Probe, SYBR Green I. 7. Temperature Gradient: With 8 dynamic temperature gradient PCR function. 8. Maximum Block Ramp Rate: 5 °C／s； 9. Temperature Range: 0-100 °C; Temperature Accuracy: ±0.2 °C; Temperature Uniformity: ±0.4 °C. 10. Dynamic Range: no less than 10 orders of magnitude. 11. Sensitivity: to detect single copy gene in human genome. |
Applications and Uses | The fluorescent quantitative PCR is widely used, which can be used in ： 1. Absolute quantification of nucleic acids (DNA or RNA) 2. Analysis of gene expression level 3. detection of gene mutation 4. GMO detection and product specificity analysis 5. Genotyping: for example, SNP detection, methylation detection and other research areas. |
Contacts | Xiaofang Tan |
Contact number | 65884616 |
Editor: Xiaofang Tan