Qingyu Wu, M.D., Ph.D.

199 Ren'ai Rd. Suzhou, Jiangsu 215123, P.R.China
Cyrus Tang Hematology Center, Soochow University
Phone: 86-512-65880877
Fax: 86-512-65880929
Email: wuqy@suda.edu.cn

Education:

1978-1982 M.D., Suzhou Medical College
1982-1985 M.S., Suzhou Medical College
1984-1988 Ph.D., Human Biology, University Paris VII
1990-1990 Postdoctoral Fellow, Washington University School of Medicine in St. Louis, USA

Professional Experience:

1994-1995 Lecturer, Washington University School of Medicine, USA
1995-1998 Scientist, Berlex Biosciences in California, USA
1998-2005 Senior Scientist, Berlex Biosciences in California, USA
2006-Present Professor, Lerner Research Institute, Cleveland Clinic, USA
2006- Present Professor, Soochow University, China

Research:

Role of cardiovascular proteases in regulating vascular and cardiac function
Corin is an important protease that regulates blood pressure and cardiac function. In the heart, corin activates natriuretic peptides, thereby promoting natriuresis, diuresis and vasodilation. This mechanism is critical in preventing hypertension and heart disease. Currently, we are studying human plasma soluble corin and its role in the diagnosis and treatment of heart failure. We also are studying corin activation mechanism and genetic mutations and/or single nucleotide polymorphisms in patients with hypertension and congestive heart failure. Our goals are to understand the biology of corin and its role in hypertension and heart disease. Our lab also studies other proteases that play a role in thrombosis, hemostasis and vascular biology.

Investigators:

Staff

Ningzheng Dong, M.D., Ph.D., Investigator

Meng Liu, Ph.D., Associate Investigator

Tiantian Zhou, Ph.D., Associate Investigator

Graduate Students

Ph.D. Students: Yayan Niu，Xianrui Zhang

Master Students: Yue Chen, Shijin Sun, Yikai Zhang, Xiabin Gu, Chunyu Du, Wenguo Li

Representative papers:

1. Wang C, Wang Z, He M, Zhou T, Niu Y, Sun S, Li H, Zhang C, Zhang S, Liu M, Xu Y, Dong N*, Wu Q*. Krüppel-like factor 17 upregulates uterine corin expression and promotes spiral artery remodeling in pregnancy. Proc Natl Acad Sci U S A. 2020;117:19425-34.
 
2. Zhang C, Zhang Y, Zhang S, Wang Z, Sun S, Liu M, Chen Y, Dong N*, Wu Q*. Intracellular autoactivation of TMPRSS11A, an airway epithelial transmembrane serine protease. J Biol Chem. 2020: 295:12686-96.
 
3. He M, Zhang Y, Li H, Liu M, Dong N*, Wu Q*. A common CORIN variant in hypertension reduces corin intracellular trafficking by exposing an inhibitory N-terminus. Biochem Biophys Res Commun. 2020;530:35-41.
 
4. Sun S, Wang L, Zhang S, Zhang C, Chen Y, Wu Q, Dong N. N-glycan in the scavenger receptor cysteine-rich domain of hepsin promotes intracellular trafficking and cell surface expression. Int J Biol Macromol. 2020;161:818-27.
 
5. Wang L, Zhang C, Sun S, Chen Y, Hu Y, Wang H, Liu M1, Dong N*, Wu Q*. Autoactivation and calpain-1-mediated shedding of hepsin in human hepatoma cells. Biochem J. 2019;476:2355-69. 
 
6. Yan R, Liu M, Hu Y, Wang L, Wang C, Jiang Y, Zhou Q, Qi X, Dong N*, Wu Q*. Ectopic expression of human airway trypsin-like protease 4 in acute myeloid leukemia promotes cancer cell invasion and tumor growth. Cancer Med. 2019 2019;8:2348–59.
 
7. Hu Y, Li H, Yan R, Wang C, Wang Y, Zhang C, Liu M, Zhou T, Zhu W, Zhang H, Dong N*, Wu Q*: Increased neutrophil activation and plasma DNA levels in patients with preeclampsia. Thromb Haemost 2018;118:2064-73.
 
8. Hu Y, Yan R, Zhang C, Zhou Z, Liu M, Wang C, Zhang H, Dong L, Zhou T, Wu Y, Dong N, Wu Q. High-Mobility Group Box 1 From Hypoxic Trophoblasts Promotes Endothelial Microparticle Production and Thrombophilia in Preeclampsia. Arterioscler Thromb Vasc Biol 2018;38:1381-91.
 
9. Zhou H, Zhu J, Liu M, Wu Q, Dong N.  Role of the protease corin in chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med. 2018;12:973-82.
 
10. Zhang Y, Zhou T, Niu Y, He M, Wang C, Liu M, Yang J, Zhang Y, Zhou J, Fukuda K, Qin J, Dong N, Wu Q. Identification and functional analysis of CORIN variants in hypertensive patients. Hum Mutat 2017;38:1700-10.
 
11. Chen S, Wang H, Li H, Zhang Y, Wu Q.  Functional analysis of corin protein domains required for PCSK6-mediated activation. Int J Biochem Cell Biol 2017;94:31-39.
 
12. Zhang Z, Hu Y, Yan R, Dong L, Jiang Y, Zhou Z, Liu M, Zhou T, Dong N, Wu Q: The transmembrane serine protease HAT- like 4 is important for epidermal barrier function to prevent body fluid loss. Sci Rep 2017;7:45262.
 
13. Li H, Zhang Y, Wu Q: Role of corin in the regulation of blood pressure. Curr Opin Nephr Hyertens 2017;26:67-73.
 
14. Dong L, Wang H, Dong N, Zhang C, Xue B, Wu Q:Localization of corin and atrial natriuretic peptide in human renal segments. Clin Sci (Lond) 2016;130:1655-64.
 
15. Chen S, Cao P, Dong N, Peng J, Zhang C, Wang H, Zhou T, Yang J, Zhang Y, Martelli EE, Naga Prasad SV, Miller RE, Malfait AM, Zhou Y, Wu Q. PCSK6-mediated corin activation is essential for normal blood pressure. Nat Med 2015;21:1048-53.
 
16. Zhang Y, Li H, Zhou J, Wang A, Yang J, Wang C, Liu M, Zhou T, Zhu L, Zhang Y, Dong N, Wu Q. A corin variant identified in hypertensive patients that alters cytoplasmic tail and reduces cell surface expression and activity. Sci Rep 2014;4:7378.
 
17. Dong N, Zhou T, Zhang Y, Liu M, Li H, Huang X, Liu Z, Wu Y, Fukuda K, Qin J, Wu Q. Corin mutations K317E and S472G from preeclamptic patients alert zymogen activation and cell surface targeting. J Biol Chem 2014;289:17909-16.
 
18. Sun S, Liu Z, Zhou H, Li G, Liu M, Yao J, Zhou T, An G, Wu Q*, Dong N*. The Role of Fucosylation in the Promotion of Endothelial Progenitor Cells in Neovascularization and Bone Repair. Biomaterials 2014;35:3777-85.
 
19. Gao L, Liu M, Dong N, Jiang Y, Lin C-Y, Huang M, Wu D, Wu Q. Matriptase is highly up-regulated in chronic lymphocytic leukemia and promotes cancer cell invasion. Leukemia 2013;27:1191-4.
 
20. Fang C, Shen L, Dong L, Liu M, Shi S, Dong N, Wu Q. Reduced urinary Corin levels in patients with chronic kidney disease. Clin Sci (Lond) 2013;124:709-17.
 
21. Dong N, Fang C, Jiang Y, Zhou T, Liu M, Zhou J, Shen J, Fukuda K, Qin J, Wu Q. Corin mutation R539C from hypertensive patients impairs zymogen activation and generates inactive alternative ectodomain fragment. J Biol Chem 2013;288:7867-74.
 
22. *Cui Y, *Wang W, *Dong N, *Lou J, Srinivasan D, Cheng W, Huang X, Liu M, Fang C, Peng J, Chen S, Wu S, Liu Z, Dong L, Zhou Y, Wu Q. Role of corin in trophoblast invasion and uterinespiral artery remodeling in pregnancy. Nature 2012;484:246-50.*Co-first authors
 
23. Qi X, Jiang J, Zhu M, Wu Q. Human corin isoforms with different cytoplasmic tails that alter cell surface targeting. J Biol Chem 2011;286:20963-9.
 
24. Dong N, Chen S, Yang J, He L, Liu P, Zheng D, Li L, Zhou Y, Ruan C, Plow E, Wu Q. Plasma soluble corin in patients with heart failure. Circ Heart Fail 2010;3:207-11.
 
25. Bugge T, Antalis T, Wu Q. Type II transmembrane serine proteases. J Biol Chem 2009;284:23177-81.
 
26. Wang W, Liao X, Fukuda K, Knappe S, Wu F, Dries DL, Qin J, Wu Q. Corin variant associated with hypertension and cardiac hypertrophy exhibits impaired zymogen activation and natriuretic peptide processing activity. Circ Res 2008;103:502-8.
 
27. Liao X, Wang W, Chen S, Wu Q. Role of glycosylation in corin zymogen activation. J Biol Chem 2007;282:27728-35.
 
28. Chan J, Knudson O, Wu F, Morser J, Dole WP, Wu Q. Hypertension in mice lacking the pro-atrial natriuretic peptide convertase, corin. Proc Natl Acad Sci USA 2005;102:785-90.
 
29. Wu F, Wu Q. Corin-mediated processing of pro-atrial natriuretic peptide in human small cell lung cancer cells. Cancer Res 2003;63:8318-22.
 
30. Yan W, Wu F, Morser J, Wu Q. Corin, a transmembrane cardiac serine protease, acts as a pro-atrial natriuretic peptide converting enzyme. Proc Natl Acad Sci USA 2000;97:8525-29.